Journal: Respiratory Research

Article Title: Differential proteins from EVs identification based on tandem mass tags analysis and effect of Treg-derived EVs on T-lymphocytes in COPD patients

doi: 10.1186/s12931-024-02980-2

Figure Lengend Snippet: Network pharmacology analysis

Article Snippet: The antibodies used in this experiment were listed as follow: BTRC Antibody (DF6534, Affinity; 1:1000); NCOA3 Antibody (AF4055, Affinity; 1:1000); TRIM28 Antibody (Bioss, bs-3581R; 1:1000); CD209 Antibody (DF2295 Affinity; 1:1000); SSR3 Antibody (DF2778, Affinity; 1:1000); Anti-rabbit IgG, HRP-linked Antibody (7074, CST; 1:6000); Vinculin Antibody (AF5122, Affinity; 1:1000); GAPDH Antibody (10494-1-AP, Proteintech; 1:10000).

Techniques:

Journal: Respiratory Research

Article Title: Differential proteins from EVs identification based on tandem mass tags analysis and effect of Treg-derived EVs on T-lymphocytes in COPD patients

doi: 10.1186/s12931-024-02980-2

Figure Lengend Snippet: Relative expressions of potential biomarkers. ( A-E ) The expression levels of ( A ) BTRC, ( B ) NCOA3, ( C ) TRIM28 ,( D ) CD209, and ( E ) SSR3. ( F ) Protein bands. \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^{\blacktriangle}$$\end{document} p < 0.05, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^{\blacktriangle\blacktriangle}$$\end{document} p < 0.01 vs. healthy control

Article Snippet: The antibodies used in this experiment were listed as follow: BTRC Antibody (DF6534, Affinity; 1:1000); NCOA3 Antibody (AF4055, Affinity; 1:1000); TRIM28 Antibody (Bioss, bs-3581R; 1:1000); CD209 Antibody (DF2295 Affinity; 1:1000); SSR3 Antibody (DF2778, Affinity; 1:1000); Anti-rabbit IgG, HRP-linked Antibody (7074, CST; 1:6000); Vinculin Antibody (AF5122, Affinity; 1:1000); GAPDH Antibody (10494-1-AP, Proteintech; 1:10000).

Techniques: Expressing, Control

Journal: The Journal of Clinical Investigation

Article Title: Short telomere syndromes cause a primary T cell immunodeficiency

doi: 10.1172/JCI120216

Figure Lengend Snippet: (A) Heatmap and dendrogram of gene expression showing the mean subtracted expression values on a log2 scale. For each of 12 samples, YC, ST, and OA groups (2 male/2 female/group), the log2 expression value was subtracted from the mean log2 expression value of the entire cohort. The dendrogram showing relatedness of the samples is above, and relatedness of the gene transcripts is to the left. The differential change in gene expression is shown as positive and negative change on color scale indicated in key. (B) Venn diagram shows 4 of 20 nonoverlapping upregulated pathways in IPA involved in apoptosis. (C and D) CD95 expression in CD4+ and CD8+ T cells, respectively. (E and F) PD-1 expression in CD4+ and CD8+ T cells, respectively. For C–F, n = 5 YC, 2 male/3 female; n = 6 ST, 2 male/4 female; and n = 5 OA, 3 male/2 female. (G and H) Kap1 and p-Kap1 levels on protein from isolated mouse T splenocytes. p-Kap1 and actin were detected first. Then the blot was stripped and reblotted with Kap1 antibody. Protein from irradiated splenocytes is a positive control. (H) Shown are quantification data from 3 independent Western blots from a total of 11 mice: WT (30 weeks, 1 male/3 female), mTR–/–G4 (30–33 weeks, 4 female), and old WT mice (50–73 weeks, 3 female). (I) qRT-PCR from unstimulated T splenocytes. Each data point represents an independent experiment with ages similar to those in H. (J) Model of T cell–aging mechanisms showing differences in immunophenotype and T cell apoptosis program in young ST T cells and OA with normal TL. Older individuals with short telomeres are predicted to have extrinsic and intrinsic apoptotic mechanisms contributing. Error bars represent SEM. *P < 0.05; **P < 0.01, Student’s t test.

Article Snippet: The following primary antibodies were used: Kap1 (rabbit, catalog A300-274, 1:1000; Bethyl Laboratories) and p-Kap1 S824 (rabbit, catalog A300-767, 1:500; Bethyl Laboratories) with acting loading control (mouse, catalog ab8226, 1:2000; Abcam).

Techniques: Expressing, Isolation, Irradiation, Positive Control, Western Blot, Quantitative RT-PCR